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Доповіді НАН України. – 2008. – N 4. – С. 176–181.
Властивості позаклітинної фруктозо-1,6-бісфосфатази Вacillus subtilis
О.В. Ястребова, Л.П. Панченко, І.Г. Скрипаль
Abstract
Extracellular fructose bisphosphatase (FBFase) (EC 3.1.3.11) from
spore-forming bacterium Bacillus subtilis is obtained. The enzyme is
inactivated in the absence of Mg2+ or Mn2+. Maximum activity of
the enzyme is observed with substrate at the 2mM concentration. AMP inhibits
FBFase activity, but the presence of PEP reduces this effect. Heavy metal
ions at a concentration of 0.1 mM in the presence of Mg2+ are not
inhibitors. Effect of Zn2+ ions on the enzyme activity is different
depending on pH and the presence of Mg2+ and EDTA. Monovalent cations
are insignificant effectors of extracellular FBFase only at their low
concentrations. The subunit size determined by SDS-PAGE analysis
was 63 kDa, which was confirmed by gel-filtration (230 kDa).
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