 |
Доповіді НАН України. – 2007. – N 9. – С. 131–136.
Дифференцированная циркадианная реакция на свет, проявляемая Т клетками и большими гранулярными лимфоцитами человека in vitro
Н.Ф. Гамалея, Е.Д. Шишко
Abstract
Recently we have shown that rhythmic daily fluctuations of the human blood
lymphocyte ability to react with sheep erythrocytes (ER activity) persisted
in lymphocytes placed in culture under the dark conditions or day-time
illumination. If the illumination was switched over to the night-time,
the inversion of this circadian rhythm was observed. The results implied that
lymphocytic cells contained a self-sustained light-responsive circadian
clock. As ER activity is characteristic of two lymphocyte subpopulations in
human blood-T cells and large granular lymphocytes (LGL), we now studied the
found circadian clock in enriched fractions of these lymphocyte
subpopulations, isolated and maintained in culture separately. It was
established that although circadian variations of the lymphocyte activity
were evident in both cell fractions, the rhythms turned out
light-entrainable only in the LGL one. However, when the two lymphocyte
subpopulations were grown in a double-chambered vessel which allowed humoral
exchange between them, T cell rhythms also became light-responsive,
suggesting that a diffusible element of the photoreceptive/circadian system
could be transferred from LGL onto T lymphocytes through a half-permeable
membrane. The findings are discussed in the context of an apparent key role
of the LGL in the proposed mechanism of non-visual circadian
photoregulation in man.
Повний текст статі в pdf-форматі
| Назад до номера | Вибір номера | Головна сторінка журналу | Головна сторінка Порталу |